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• 產(chǎn)品描述： Ribociclib (LEE011) succinate (Kisqali) 是一種高度特異性的針對 CDK4 和 CDK6 的雙重抑制劑，對應的IC50值分別為10 nM和39 nM。

• 靶點： CDK4(Cell-free assay):10 nM;CDK6(Cell-free assay):39 nM;CDK

• 體內(nèi)研究：
  LEE011 (200 mg/kg daily, p.o.) significantly causes tumor growth delay in mice harboring the BE2C or 1643 xenografts with no weight loss or other signs of toxicity.

• 細胞實驗： Cell lines: BE2C, IMR5, 1643, SY5Y, CHP134, SKNSH, NGP, KELLY, LAN5, NLF, NB69, SKNDZ, NBSD, NBLS, SKNFI, EBC1, SKNAS, NB16, RPE1 cell lines Concentrations: 10 μM Incubation Time: ~100 h Method: A panel of neuroblastoma cell lines, selected based upon prior demonstration of substrate adherent growth, is plated in triplicate on the Xcelligence Real-Time Cell Electronic Sensing system and treated 24 hours later with a four-log dose range of inhibitor or with a dimethyl sulfoxide (DMSO) control. Cell indexes are monitored continuously for ~100 hours, and IC50 values are determined as follows: growth curves are generated by plotting the cell index as a function of time and are normalized to the cell index at the time of treatment for a baseline cell index of 1. The area under the normalized growth curve from the time of treatment to 96 hours posttreatment is then calculated using a baseline area of 1 (the cell index at the time of treatment). Areas are normalized to the DMSO control, and the resulting data ar

• 動物實驗： Animal Models: Mice bearing BE2C, NB-1643, or EBC1 xenografts Dosages: ~200 mg/kg daily Administration: p.o.

• 參考文獻：
  1. Todd VanArsdale, et al. Clin Cancer Res. 2015 Jul 1;21(13):2905-10. 2. Rader J, et al. Clin Cancer Res. 2013, 19(22), 6173-6182. 3. Antonio Marra, Giuseppe Curigliano. NPJ Breast Cancer. 2019 Aug 29;5:27.

• 溶解性： Soluble  in  DMSO

• 保存條件： -20°C;充氬

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	1.81 ml	9.048 ml	18.095 ml
  5 mM	0.362 ml	1.81 ml	3.619 ml
  10 mM	0.181 ml	0.905 ml	1.81 ml
  50 mM	0.036 ml	0.181 ml	0.362 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

輸入產(chǎn)品批號：

本計算器可幫助您計算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *